CloneID: hTFFab148H
Antigen Long Description: The original antibody was isolated from a human Fab antibody library generated from peripheral blood collected from 300 healthy individuals.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P13726
Specificity Statement: This antibody binds human tissue factor (TF) a hemostatic protein which is involved in the extrinsic pathway of coagulation. TF is receptor for circulating serine protease factor VIIa, activating the catalytic VIIa protease domain. The complex formed functions to activate the zymogen factor X into activated factor Xa, which then catalyzes the formation of Prothrombin to Thrombin. The pathways results in a fibrin clot which acts to stop bleeding. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Application Notes (Clone): The binding characterization of this Fab antibody was done using ELISA and dot blot. This antibody shows positive plasma coagulation inhibitory activity. This antibody is capable of neutralizing human tissue factor in vitro by inhibiting the binding of TF factor/Vlla complex to factor X. The inhibition rate increase with the concentration. The pharmacodynamic and pharmacokinetic activity of this antibody was studied in vivo in rat models (CN101423552).
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