CloneID: 132-D4
Antigen Long Description: The original antibody was generated by immunizing llamas with a cocktail of murine and human Toll-like receptors (TLRs) that contained recombinant human TLR4/MD2, recombinant human TLR3, recombinant murine TLR3, and recombinant murine TLR2/Fc chimera. Peripheral blood mononuclear cells were isolated from the animals' blood for the construction of a phage display library, and the library was panned against the TLRs used for immunization.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a chimeric antibody, developed from the original camelid VHH antibody, specifically engineered for enhanced compatibility with existing reagents, assays, and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q99MB1
Specificity Statement: This antibody is specific for mouse TLR3.
Application Notes (Clone): The binding specificity of the original version of this antibody (VHH) was tested using ELISA. In a cell-based neutralizing assay with mTLR3-overexpressing HEK cells, an antagonistic effect was observed, as measured by NFκB expression after cell lysis compared to the control group (WO2009138494A2).
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