Anti-VACV (SC212)

Anti-VACV [SC212], Recombinant, IgG lambda, Rabbit

CloneID: SC212

Antigen Long Description: A phage display library of human single chain variable fragment (scFv) antibodies was constructed from the B cells of healthy volunteers boosted by the VACV vaccine. Human scFv was isolated by panning against VACV.

Buffer Composition: PBS only.

Chimeric Use Statement: This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.

Specificity Statement: The antibody is specific for VACV.

Application Notes (Clone): The scFv fragment was converted to human IgG1 (VC212). The binding affinity of VC212 was estimated by competition ELISA to IC50 values 22 µg/ml (146.67 nM). The humanized version of the antibody neutralized the VACV, as evidenced by the plaque reduction neutralization test result with a PRNT50 of ~0.16 mg/ml (~1.07 µM) (Shin et al., 2019; PMID: 30856707).

• SKU: ABAAb03517-23.0-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03517-23.0-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Virus
• Clonality: Recombinant
• Application: ELISA
• Isotype: IgG lambda
• Host: Rabbit