CloneID: SC212
Heavy Chain modification: ScFv
Antigen Long Description: A phage display library of human single chain variable fragment (scFv) antibodies was constructed from the B cells of healthy volunteers boosted by the VACV vaccine. Human scFv was isolated by panning against VACV.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: The antibody is specific for VACV.
Application Notes (Clone): The scFv fragment was converted to human IgG1 (VC212). The binding affinity of VC212 was estimated by competition ELISA to IC50 values 22 µg/ml (146.67 nM). The humanized version of the antibody neutralized the VACV, as evidenced by the plaque reduction neutralization test result with a PRNT50 of ~0.16 mg/ml (~1.07 µM) (Shin et al., 2019; PMID: 30856707).
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