CloneID: 2H1
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was raised by panning VEGF against a recombinant human antibody library containing 4.25 × 108 scFvs from non-immunized healthy donors^.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human scFv format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q9UNS8
Specificity Statement: This amtibody is specific for the human VEGF protein.
Application Notes (Clone): The original antibody was used for surface plasmon resonance on VEGF. This showed the binding affinity of the antibody to its antigen. This antibody was tested in vitro. It was assessed in a VEGF-stimulated HUVEC mitogenesis, it showed it was able to inhibit HUVEC proliferation in a dose dependent matter. This antibody was used for inhibition of VEGF-KDR binding. It showed that it was able to inhibit the binding. This antibody was used for an ELISA on different peptides of its antigen to determine its epitope (Lamdan et al., 2011; PMID:21167224, Lamdan et al., 2013; PMID:23702826).
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