CloneID: 82D6A3
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was purified from murine ascites by Protein A chromatography.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted mouse IgG1 Fc Silent™ antibody, based on the original mouse IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P04275
Specificity Statement: This antibody is specific for human vWF, A3 domain.
Application Notes (Clone): This antibody was used as an inhibitor to study platelet adhesion to collagen VI and ECM. It was found to inhibit the binding of von Willebrand factor (VWF) to collagen I and III (Hoylaerts et al., 1997; PMID: 9164855). This antibody was found to have a comparable affinity for baboon vWF as for human vWF and inhibited the binding of baboon vWF to collagen with an inhibitory concentration of 50% (IC50) of 3.5 μg/ml. Furthermore, it prevented the binding of VWF to collagen and removed bound VWF from a collagen surface, albeit with a lower efficacy. Moreover, the antithrombotic efficacy of the antibody was evaluated in a modified Folts model in baboons; this antibody was used to inhibit the interaction between collagen and vWF to prevent arterial thrombosis in baboons (Wu et al., 2002; PMID: 11986216). The crystal structure of the antibody's Fab fragment, in complex with the vWF A3 domain, was determined (Staelens et al., 2006; PMID: 16314412).
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