CloneID: FA7
Antigen Long Description: This antibody was generated by panning a scFv library from BALB/c mice immunized with purified fusion protein against GST-VP1694 antigen.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This full-length, chimeric human antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q87P24
Specificity Statement: This antibody is specific for the VP1694 protein from Vibrio parahaemolyticus. This proteins is necessary for this bacteria to act as a pathogen. Vibrio parahaemolyticus can cause gastroenteritis in humans.
Application Notes (Clone): An ELISA was performed on associated V. parahaemolyticus proteins (TDH, TLH, VopQ, VP1694), Homologous protein HrpA, and PBS using the scFv version of this antibody. It showed a stronger binding to VP1694 than to other proteins. Furthermore, a western blot was performed on VP1694 using this antibody. The western blot showed the binding of VP1694 by the antibody (Wang et al, 2014; pmid:25320414).
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