CloneID: Mab 216
Antigen Long Description: The original antibody was generated by immunizing female WKY/NCrj rats with crude virus preparations and hybridomas were generated using cells from the enlarged medial iliac lymph nodes.
Origin Pub PMID: 19108623
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q9ICB7
Specificity Statement: This antibody binds the VP28 envelope protein of the White spot syndrome virus (WSSV). White spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. VP28 is one of the most important envelope proteins of WSSV.
Application Notes (Clone): This antibody was capable of detecting VP28 protein of WSSV in a western blot. Immuno-electron microscopy further showed that the epitope of this antibody is located on the viral envelope protein. In vitro neutralization studies in shrimp primary lymphoid cell culture showed diminished infectivity. In vivo neutralization assay showed 80% survival in shrimps injected with WSSV at a dilution of 1 × 10−3 pre-incubated with purified immunoglobulin (IgG) version of MAb 216. At higher virus concentration this antibody was not able to neutralize the virus (K.D.T. Natividad et al., 2007).
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