CloneID: QY1.5
Antigen Long Description: The original antibody was generated using phage display technology by panning an scFv library against zearalenone-ovalbumin conjugate. The scFv library was created by linking the heavy and light chain genes of antizearalenone hybridoma cell line (2G3-6E3-2E2) using DNA linker encoding peptide (Gly4Ser)3 and cloning into a phagemid (pCANTAB5E) to be expressed as a fusion protein on the surfaces of recombinant phages. The original hybridoma (2G3-6E3-2E2) was generated using zearalenone-BSA conjugate as an immunogen.
Buffer Composition: PBS only.
Chimeric Use Statement: This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody is specific for zearalenone and show some cross reactivity with hydroxylated zearalenone analogs. It is a mycotoxin produced mainly by fungi belonging to the genus Fusarium in foods and feeds. Possess estrogenic activity in pigs, cattle and sheep, with low acute toxicity. When fed to animals, the compound causes hypoestrogenism with symptoms such as enlargement of the uterus and nipples, vulvar swelling, vaginal prolapse, and infertility
Application Notes (Clone): The binding specificity of this scFv antibody towards mycotoxin zearalenone was determined using ELISA. The scFv version of this antibody was also able to detect zearalenone in spiked corn extract in a competitive-indirect ELISA (PMID: 8979354).
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