CloneID: G9E
Antigen Long Description: This antibody was generated using 6XL method (Kwakkenbos et al., 2010). Memory B cells were isolated from a subject previously infected with ZIKV. After identification of ZIKV-specific monoclonal cultures, genetic material of the variable domains was isolated and recombinant IgG1 lambda antibody forms were grafted with the sequences.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody binds to ZIKV and does not cross-react with DENV4, which was tested as a representative DENV serotype. Its epitope is distinct from the A9E anti-ZIKV antibody.
Application Notes (Clone): This antibody has very strong neutralizing properties against Asian and African lineages of ZIKV (Collins et al., 2019). Furthermore, this antibody was effective in preventing Zika infection when administered from one day before the infections in mice (Collins et al., 2019). Therefore, A9E is recommended in development of diagnostic assays which differentiate ZIKV from other flaviviruses (it does not crossreact with DENV) and in the development of therapeutics and vaccines against ZIKV.
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