CD106 / VCAM1(B-K9), CF740 conjugate, 0.1mg/mL

Description: CD106 (also known as vascular cell adhesion molecule-1, or VCAM-1) is a member of the Ig superfamily of adhesion molecules. It is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte beta-1 integrin VLA-4 and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: B-K9

Immunogen: Activated human umbilical vein endothelial cells (HUVEC)

Antibody Reactivity: CD106/VCAM-1

References: Note: References for this clone sold by other suppliers may be listed for expected applications.

1. J Gen Virol (2016) 97(11):2989-3006. (Flow, functional studies)
2. Am J Physiol Lung Cell Mol Physiol (2002) 282: L1279-L1288. (functional studies)
3. Endocrinology (2003) 144(1): 360-370. (Flow)

Entrez Gene ID: 7412

Expected AB Applications: Flow, surface (published for clone)/Functional studies (published for clone)/IF (expected)

Z-Antibody Applications: Flow, surface (published)/Functional studies (published)/IF (expected)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user

• SKU: BTMBNC740304-500
• Manufacturer: Biotium
• Manufacturer SKU: BNC740304-500
• Package Unit: 500 uL
• Quantity Unit: STK
• Reactivity: Human
• Clonality: Monoclonal
• Application: Immunofluorescence, Flow Cytometry, Functional Studies
• Isotype: IgG1 kappa
• Host: Mouse
• Conjugate: Conjugated, CF740