Description: Recognizes a protein of 180 kDa, identified as CD11a (Leucocyte Workshop II; Code N202). CD11a complex with the 2 subunit of the integrin family, CD18, to form the cell surface heterodimer, LFA-1 or CD11a /C18 (aLbL). LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: Cris-3
Immunogen: Stimulated human leukocytes
Antibody Reactivity: CD11a
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Knapp W. et al. Leukocyte Typing IV, Oxford Unioversity Press, Oxford, 1989.
- Reinherz EL et al. eds. Leukocyte Typing II, Oxford University Press, Oxford, 1985.
- J Exp Med (1990) 172: 335-345. (Flow, surface)
- Am J Physiol Cell Physiol (1998) 274(6): C1634-C1644. (receptor blockade).
Entrez Gene ID: 3683
Expected AB Applications: Functional studies (published for clone)
Z-Antibody Applications: Flow (verified)/Functional studies (published)
Verified AB Applications: Flow (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL
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