CD20(93-1B3), CF740 conjugate, 0.1mg/mL

Description: Recognizes a protein of 30-33 kDa, which is identified as CD20 (Workshop V; Code CD20.4). It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as calcium channel involved in B cell activation and cell cycle progression.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: 93-1B3

Immunogen: Stimulated human leukocytes

Antibody Reactivity: CD20

References: Note: References for this clone sold by other suppliers may be listed for expected applications.

1. J Leukoc Biol (2001) 69: 215-223. (cell enrichment)
2. Exp Oncol (2004) 26(3):210-216. (IF)

Entrez Gene ID: 931

Expected AB Applications: Flow (surface)/Functional studies (published for clone)/IF (published for clone)

Z-Antibody Applications: Flow, surface/Functional studies (published)/IF (published)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Functional Studies order Ab without BSA & azide)/Optimal dilution for a specific application should be determined by user

• SKU: BTMBNC741047-100
• Manufacturer: Biotium
• Manufacturer SKU: BNC741047-100
• Package Unit: 100 uL
• Quantity Unit: STK
• Reactivity: Human
• Clonality: Monoclonal
• Application: Immunofluorescence, Flow Cytometry, Functional Studies
• Isotype: IgG1 kappa
• Host: Mouse
• Conjugate: Conjugated, CF740