Description: Recognizes a protein of 33-37 kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: B9E9
Immunogen: Lymphoblastoid cell line Daudi
Antibody Reactivity: CD20
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Blood (2002) 100(13), 4512-4520. (surface Flow)
- Cancer Res (2000) 60, 7170 -7176. (receptor activation)
Blood (2003) 101 (7), 2507-2513. (WB)
Entrez Gene ID: 931
Expected AB Applications: Flow, surface (published for clone)/Functional studies (published for clone)/WB (published for clone)
Z-Antibody Applications: Flow, surface (published)/Functional studies (published)/WB (published)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Flow cytometry 5 uL per test per one million cells in 0.1 mL, or 5 uL per 100 uL of whole blood/Optimal dilution for a specific application should be determined by user
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