CD20 / MS4A1 (B-Cell Marker) (rIGEL/773), CF740 conjugate, 0.1mg/mL

CD20 / MS4A1 (B-Cell Marker) (rIGEL/773), CF740 conjugate, 0.1mg/mL
SKU
BTMBNC741829-100
Packaging Unit
100 uL
Manufacturer
Biotium

Availability: loading...
Price is loading...
Description: This antibody recognizes a protein of 30-33 kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin's disease, particularly of lymphocyte predominant type.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: rIGEL/773

Immunogen: Recombinant full-length human MS4A1 protein

Antibody Reactivity: CD20

Entrez Gene ID: 931

Z-Antibody Applications: Flow (verified)/IF (verified)/IHC, FFPE (verified)

Verified AB Applications: Flow (verified)/IF (verified)/IHC (FFPE) (verified)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Immunohistology (formalin): 0.5-1 ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user
More Information
SKU BTMBNC741829-100
Manufacturer Biotium
Manufacturer SKU BNC741829-100
Package Unit 100 uL
Quantity Unit STK
Reactivity Human
Clonality Recombinant
Application Immunofluorescence, Flow Cytometry, Immunohistochemistry
Isotype IgG2a kappa
Host Mouse
Conjugate Conjugated, CF740
Product information (PDF) Download
MSDS (PDF) Download