CD22(Myg13), CF740 conjugate, 0.1mg/mL

Description: This antibody ecognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: Myg13

Immunogen: Human Raji cells

Antibody Reactivity: BL-CAM/CD22

References: Note: References for this clone sold by other suppliers may be listed for expected applications.

1. Glia (2004) 46: 369-379. (functional studies; WB)
2. Exp Mol Pathol (2010) 88(2): 238-249. (Flow, surface)
3. J Immunol (2011) 186: 1554-1563. (cell internalization)

Entrez Gene ID: 933

Expected AB Applications: Flow, surface (published for clone)/Functional studies (published for clone)/WB (published for clone)

Z-Antibody Applications: Flow (verified)/Flow, surface (published)/Functional studies (published)/WB (published)

Verified AB Applications: Flow (verified)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user

• SKU: BTMBNC740974-100
• Manufacturer: Biotium
• Manufacturer SKU: BNC740974-100
• Package Unit: 100 uL
• Quantity Unit: STK
• Reactivity: Human, Mouse (Murine), Rat (Rattus)
• Clonality: Monoclonal
• Application: Western Blotting, Flow Cytometry, Functional Studies
• Isotype: IgG1 kappa
• Host: Mouse
• Conjugate: Conjugated, CF740