CD25/ IL2RA (Activated Lymphocyte Marker) (IL2RA/2395), CF740 conjugate, 0.1mg/mL

CD25/ IL2RA (Activated Lymphocyte Marker) (IL2RA/2395), CF740 conjugate, 0.1mg/mL
SKU
BTMBNC742395-500
Packaging Unit
500 uL
Manufacturer
Biotium

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Description: This antibody recognizes a protein of 55 kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin's disease, HTLV-1 adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R  chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex.CD25 antibodies detect three epitope regions, A, B and C. This MAb This antibody recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: IL2RA/2395

Immunogen: Recombinantfragment of human IL2RA protein (around aa 42-183) (exact sequence is proprietary)

Antibody Reactivity: CD25/IL2RA

Entrez Gene ID: 3559

Z-Antibody Applications: IHC, FFPE (verified)

Verified AB Applications: IHC (FFPE) (verified)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunohistology (formalin): 2-4 ug/mL for 30 minutes at RT/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes/Optimal dilution for a specific application should be determined by user
More Information
SKU BTMBNC742395-500
Manufacturer Biotium
Manufacturer SKU BNC742395-500
Package Unit 500 uL
Quantity Unit STK
Reactivity Human
Clonality Monoclonal
Application Immunohistochemistry
Isotype IgG2b
Host Mouse
Conjugate Conjugated, CF740
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