Description: Recognizes a protein of 33-55 kDa, identified as CD37 (Workshop V; Code CD37.7). CD37 is strongly expressed on normal and neoplastic mature (sIg ) B-lymphocytes. In B-cell ontogeny, CD37 appears after the pre-B-cell stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells.1 CD37 is also present, at low densities, on resting and activated T cells, neutrophils, monocytes, and some myelomonocytic leukemia cells. It is absent from platelets, erythrocytes. CD37 is a member of a family of tetraspan transmembrane proteins, including CD9, CD53, CD63, CD81, and CD82. It associates other tetraspan transmembrane proteins and MHC class II molecules to form a large complex at the surface of B cells and play a role in signal transduction. CD37 is a valuable and stable marker for peripheral mature B-cells and corresponding malignancies like B-cell chronic lymphocytic leukemia (B-CLL), hairy cell leukemia (HCL), and all types of B-cell non-Hodgkin'' lymphoma (B-NHL).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: IPO-24
Immunogen: Spleen cells of a patient with hairy cell leukemia
Antibody Reactivity: CD37
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Blood (2010) 116(7): 1025-1034. (IHC, FFPE; IF)
- PLoS One 12(10): e0185940. (Flow, surface; IF)
Entrez Gene ID: 951
Expected AB Applications: Flow, surface (published for clone)/IHC (FFPE) (published for clone)/IF (published for clone)
Z-Antibody Applications: Flow (verified)/Flow, surface (published)/IF (published)/IHC, FFPE (published)
Verified AB Applications: Flow (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user
English
Deutsch
