Description: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: UCHT1
Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor
Antibody Reactivity: CD3e
References: Note: References for this clone sold by other suppliers may be listed for expected applications. 1. Blood (1988) 71(3): 603-612. (immunofluorescence, western)2. J Immunol Meth (1988) 106(1): 127-133. (ELISA)3. J Exp Med (1991) 173: 7-17. (immunoprecipitation)4. J Immunol (2002) 168: 3235-3241. (functional studies)
Entrez Gene ID: 916
Expected AB Applications: ELISA (published for clone)/Functional studies (published for clone)/IP (published for clone)/WB (published for clone)
Z-Antibody Applications: Functional studies (published)/ELISA (published)/Flow, surface (verified)/IF (verified)/IP (published)/WB (published)
Verified AB Applications: Flow (surface) (verified)/IF (verified)
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