Description: Recognizes a protein of 55 kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. This MAb was characterized as human CD4 antibody at II and IV International Workshop on Human Leukocyte Differentiation Antigens.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: EDU-2
Immunogen: Stimulated human leukocytes
Antibody Reactivity: CD4
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Reinberz EL et al. eds. Leykcocyte Typing II, Springer-Verlag, Berlin, 1985.
- Knapp W. et al. (eds) Leukocyte Typing IV, p314-330, Oxford University Press, Oxford, 1989.
- Immunol Cell Biol (2015) 93: 396-405. (functional studies; binding site identification)
Entrez Gene ID: 920
Expected AB Applications: Functional studies (published for clone)
Z-Antibody Applications: Functional studies (published)/Flow, surface (verified)/IF (verified)/IHC, frozen (verified)
Verified AB Applications: Flow (surface) (verified)/IF (verified)/IHC (frozen) (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence (direct conjugate): 1-5 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Does not react with Macaca mulatta (rhesus monkey), others not tested/Optimal dilution for a specific application should be determined by user
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