Description: Recognizes a protein of 55 kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: RIV7
Immunogen: Human peripheral lymphocytes
Antibody Reactivity: CD4
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Knapp W. et al. (eds) Leukocyte Typing IV, p314-330, Oxford University Press, Oxford, 1989.
- Br J Haematol (2004) 128: 73-81. (functional studies)
Entrez Gene ID: 920
Expected AB Applications: Functional studies (published for clone)
Z-Antibody Applications: Functional studies (published)/Flow, surface (verified)/IF (verified)
Verified AB Applications: Flow (surface) (verified)/IF (verified)
Antibody Application Notes: Functional studies: Order Ab without azide; Flow cytometry: 1-2 ug/million cells in 0.1 mL; Immunofluorescence: 1-2 ug/mL; Optimal dilution for a specific application should be determined.
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