Description: Recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: UCHL-1
Immunogen: Cultured human T-cells from an IL-2-dependent T-cell line (CA1) (UCHL-1); Recombinant human CD45RO protein (T200/797)
Antibody Reactivity: CD45RO
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Immunology (1988) 64: 331-336. (immunoprecipitation)
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- J Biol Chem (1996) 271(2): 755-760.
- J Immunol (2005) 174: 2712-2719. (functional studies)
Entrez Gene ID: 5788
Expected AB Applications: Functional studies (published for clone)/IP (published for clone)/WB (published for clone)
Z-Antibody Applications: Functional studies (published)/Flow, surface (verified)/IF (verified)/IHC, FFPE (verified)/IP (published)/WB (published)
Verified AB Applications: Flow (surface) (verified)/IF (verified)/IHC (FFPE) (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Immunohistology formalin-fixed 0.25-0.5 ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user
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