Description: This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/803 or LAMP3/968 or NKI/C3 or MX-49.129.5. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: LAMP3/529
Immunogen: Recombinant human CD63 protein
Antibody Reactivity: CD63
Entrez Gene ID: 967
Z-Antibody Applications: Flow (verified)
Verified AB Applications: Flow (verified)
Antibody Application Notes: Flow cytometry: 1-2 ug/million cells/Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/ELISA: For coating order antibody without BSA/Immunofluorescence: 0.5-1 ug/mL/Optimal dilution for a specific application should be determined by user
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