Description: Recognizes a protein of 40 kDa, identified as CD7, a member of the immunoglobulin gene superfamily. Its N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: T3-3A1
Immunogen: Human T cells
Antibody Reactivity: CD7
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- PNAS USA (1979) 76: 5829-5833.
- PNAS USA (1980) 77(5): 2914-2918. (IP; functional studies)
- J Immunol Meth (1987) 96(2), 201-209. (cell ELISA)
- PNAS USA (1996) 93: 10348-10353. (cell depletion)>/li>
- Blood (1998) 91(8) 2760-2771. (Flow)
- J Cell Biol (1998) 142(5): 1245-1256. (IF; IP)
- Theranostics (2018) 8(21): 6070-6087. (mAb characterization; in vivo imaging)
Entrez Gene ID: 924
Expected AB Applications: ELISA (published for clone)/Flow, surface (published for clone)/Functional studies (published for clone)/IF (published for clone)/IP (published for clone)
Z-Antibody Applications: Flow, surface (published)/Functional studies (published)/IF (published)/ELISA (published)/IP (published)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user
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