CRE/CREB eGFP Reporter Lentivirus

Products from BPS Bioscience require a minimum order value above 400€

Description: The CRE/CREB eGFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an eGFP gene driven by a multimerized cAMP response element (CRE) located upstream of the minimal TATA promoter . After transduction, activation of the cAMP/PKA signaling pathway in the target cells can be monitored by examining eGFP expression.

Formulation: The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.

Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Supplied As: Two vials (500 µl x 2) of firefly luciferase lentivirus at a titer ≥5 x 10⁶ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Warnings: Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.Biosafety: of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Biosafety Level: BSL-2

• SKU: BPS78153
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 78153
• Package Unit: 500 µl x 2
• Quantity Unit: STK