Description: This antibody reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: C-50
Immunogen: Cytoskeletal preparation from HeLa cells
Antibody Reactivity: Cytokeratin 5/8
Entrez Gene ID: 3852 (CK5); 3856 (CK8)
Z-Antibody Applications: IHC, FFPE (verified)
Verified AB Applications: IHC (FFPE) (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunohistology formalin-fixed 0.5-1.0 ug/mL/For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/mL PBS, 15 min at RT/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Immunofluorescence 0.5-1.0 ug/mL/Western blotting 0.5-1.0 ug/mL/Does not react with chicken , or Xenopus/Optimal dilution for a specific application should be determined by user
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