Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: C-51
Immunogen: Cytoskeleton preparation from HeLa cells
Antibody Reactivity: Cytokeratin 8/18
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Brain Pathol (1999) 9: 617-626. (IHC, FFPE)
- Br J Cancer (1999) 81(5): 769-773. (WB)
- Int J Cancer (2004) 111: 662-668. (WB)
- Cell (2014) 159(1): 163-175. (IF; IHC, FFPE)
Entrez Gene ID: 3856 (CK8); 3875 (CK18)
Expected AB Applications: IF (published for clone)/WB (published for clone)
Z-Antibody Applications: IF (published)/IHC, FFPE (verified)/WB (published)
Verified AB Applications: IHC (FFPE) (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Does not react with mouse, rat, ferret, rabbit, donkey, chicken, Xenopus; others not known/Immunohistology formalin-fixed 0.5-1.0 ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Western blotting 0.5-1.0 ug/mL/Optimal dilution for a specific application should be determined by user
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