Cytokeratin 8 (KRT8) (rB22.1), CF740 conjugate, 0.1mg/mL

Cytokeratin 8 (KRT8) (rB22.1), CF740 conjugate, 0.1mg/mL
SKU
BTMBNC742175-100
Packaging Unit
100 uL
Manufacturer
Biotium

Availability: loading...
Price is loading...
Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma of the breast.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: rB22.1

Immunogen: Recombinant full-length human KRT8 protein

Antibody Reactivity: Cytokeratin 8

Entrez Gene ID: 3856

Z-Antibody Applications: IHC, FFPE (verified)/WB (verified)

Verified AB Applications: IHC (FFPE) (verified)/WB (verified)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Does not react with mouse, rat, chicken, or Xenopus laevis/Immunohistology (formalin): 0.5-1 ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes/Optimal dilution for a specific application should be determined by user
More Information
SKU BTMBNC742175-100
Manufacturer Biotium
Manufacturer SKU BNC742175-100
Package Unit 100 uL
Quantity Unit STK
Reactivity Human, Pig (Porcine), Sheep (Ovine), Rabbit, Cow (Bovine)
Clonality Recombinant
Application Western Blotting, Immunohistochemistry
Isotype IgG1
Host Mouse
Conjugate Conjugated, CF740
Product information (PDF) Download
MSDS (PDF) Download