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Application: Activation and expansion of Vδ1 T cell from freshly isolated for downstream applications in CAR (chimeric antigen receptor) γδ T cell development, cytotoxicity assays and flow cytometry.
Background: T lymphocytes are composed of two subpopulations: αβ T cells and γδ T cells. They are distinguished by the expression of either a TCR or a γδ TCR, respectively. αβ T cells are the predominant subset of T cells in peripheral blood and recognize antigens presented by MHC (major histocompatibility complex) molecules. γδ T cells are less abundant and recognize antigens independently of MHC presentation. While both αβ T cells and γδ T cells contribute to cell cytotoxicity through distinct mechanisms to target and eliminate infected or abnormal cells, γδ T cells have a lower risk of causing GvHD (Graft-versus-Host Disease) when injected into humans and have demonstrated cytotoxicity against a wide range of tumor types. γδ TCRs are cell type-specific, Vδ1 expressing T cells are found in mucosal and epithelial tissues and correspond to about 15% of the γδT cells present in PBMCs, with the % of γδ being only 5% of all the T cells. Vδ1 T cells are highly cytolytic, meaning they can effectively kill tumor cells. Engineering Vδ1 T cells to express chimeric antigen receptors (CARs) further enhances their antitumor activity and antigen specificity.
Description: Delta 1 (Vδ1) T Cell Expansion System is suitable for the ex vivo culture and expansion of human Vδ1 (delta 1, D1) T cells. γδ T cells typically make up only 1-5% of total T cells in PBMCs (peripheral blood mononuclear cells) and therefore they need to be successfully activated and expanded to obtain an adequate number of cells for γδ T cell-based immunotherapy studies. This product contains media and reagents necessary to drive the robust activation and expansion of the Vδ1 T cells subpopulation from PBMCs. Vδ1 T Cell Expansion System is provided with enough reagents and materials for the ex vivo culture and expansion of human Vδ1 T cells from a starting population of 3 x 107 PBMCs. It is possible to use this kit for multiple expansions from smaller PBMC amounts. Note: The use of this expansion system requires Anti-PE MicroBeads and MiniMACS™ Starting Kit (Miltenyi), that should be purchased separately (see information in Materials Required but not Supplied).
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. This assay kit will perform optimally for up to 6 months from the date of receipt when the materials are stored as directed.
Supplied As: Vδ1 T Cell Expansion System is provided with enough reagents and materials for the ex vivo culture and expansion of human Vδ1 T cells from a starting population of 3 x 107 PBMCs.
Target: T Cell
Warnings: This product is for research purposes only and not for human or therapeutic use. This product should be considered hazardous and is harmful by inhalation, in contact with skin, eyes, clothing, and if swallowed. If contact occurs, wash thoroughly.Donors have been screened and determined negative for: / Hepatitis B (anti-HBc EIA, HBsAg EIA) / Hepatitis C (anti-HCV EIA) / Human Immunodeficiency Virus (HIV-1/HIV-2 plus O) / Human T-Lymphotropic Virus (HTLV-I/II) / HIV-1/HCV/HBV / West Nile Virus / Trypanasoma cruzi
Biosafety Level: BSL-1
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