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Application: Activation and expansion of Vδ2 T cell from freshly isolated for downstream applications in CAR (chimeric antigen receptor) γδ T cell development, cytotoxicity assays and flow cytometry.
Background: T lymphocytes are composed of two subpopulations: αβ T cells and γδ T cells. They are distinguished by the expression of either a TCR or a γδ TCR, respectively. αβ T cells are the predominant subset of T cells in peripheral blood and recognize antigens presented by MHC (major histocompatibility complex) molecules. γδ T cells are less abundant and recognize antigens independently of MHC presentation. While both αβ T cells and γδ T cells contribute to cell cytotoxicity through distinct mechanisms to target and eliminate infected or abnormal cells, γδ T cells have a lower risk of causing GvHD (Graft-versus-Host Disease) when injected into humans and have demonstrated cytotoxicity against a wide range of tumor types. γδ TCRs are cell type-specific, with Vγ9Vδ2 being present in T cells and corresponding to about 5% of the T cell population in blood. Vγ9Vδ2 T cells are involved mostly in immune responses to pathogens and long-term modulation of inflammation, and can recognize nonpeptide phosphor-antigens, alkylamines and synthetic amino-bisphosphonates. Vγ9Vδ2 T cells are being studied for the treatment of solid tumors and hematological disorders and are becoming a highly promising cancer therapy. Further studies on how best to utilize Vγ9Vδ2 T cells, and methods to enhance their presence, will open new therapeutic avenues for cancer and infections.
Description: Delta 2 (Vδ2) T Cell Expansion System is suitable for the ex vivo culture and expansion of human Vδ2 (delta 2, D2) T cells. γδ T cells typically make up only 1-5% of total T cells in PBMCs (peripheral blood mononuclear cells) and therefore they need to be successfully activated and expanded to obtain an adequate number of cells for γδ T cell-based immunotherapy studies. This product contains media and reagents necessary to drive the robust activation and expansion of the Vδ2 T cells subpopulation from PBMCs. Vδ2 T Cell Expansion System is provided with enough reagents and materials for the ex vivo culture and expansion of human Vδ2 T cells from a starting population of 3 x 107 PBMCs. It is possible to use this kit for multiple expansions from smaller PBMC amounts.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. This assay kit will perform optimally for up to 6 months from the date of receipt when the materials are stored as directed.
Target: Delta 2 T Cells
Warnings: This product is for research purposes only and not for human or therapeutic use. This product should be considered hazardous and is harmful by inhalation, in contact with skin, eyes, clothing, and if swallowed. If contact occurs, wash thoroughly. Donors have been screened and determined negative for:Hepatitis B (anti-HBc EIA, HBsAg EIA)Hepatitis C (anti-HCV EIA)Human Immunodeficiency Virus (HIV-1/HIV-2 plus O)Human T-Lymphotropic Virus (HTLV-I/II)HIV-1/HCV/HBVWest Nile VirusTrypanasoma cruzi
Biosafety Level: BSL-1
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