Description: This antibody recognizes a protein of 130 kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (>80%) and specificity (100%) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: 5H10
Immunogen: Recombinant human DSG3 extracellular protein fragment (exact sequence is proprietary)
Antibody Reactivity: Desmoglein-3
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Clin Invest (1998) 102:775-782. (WB)
- J Inv Dermatol (2002) 118(5): 845-850. (IF)
- PLoS One 5(12): e14211. (IP)
- J Cell Sci (2003) 116: 4239-4248. (Flow, surface)
Entrez Gene ID: 1830
Expected AB Applications: Immuofluorescence (published for clone)/Flow, surface (published for clone)/IP (published for clone)/WB (published for clone)
Z-Antibody Applications: Flow, surface (published)/IF (published)/IP (published)/WB (published)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Western blotting 0.5-1 ug/mL/Optimal dilution for a specific application should be determined by user
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