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Application: Screen small molecule inhibitors or antagonists that affect ATPase activity of DHX9 in high throughput screening (HTS) applications.
Background: DHX9 (DExH-Box Helicase 9), also known as RNA Helicase A or Nuclear Helicase II (NDHII), is a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes mainly to the nucleus but can migrate to the cytoplasm, and functions as a transcriptional regulator. It is ubiquitously expressed and abundant. It interacts with many proteins, such as PRMT1 (protein arginine N-methyltransferase 1) and WRN (Werner Syndrome ATP-dependent Helicase). This protein may also be involved in the expression and nuclear export of retroviral RNAs, and is studied for its roles in cancer progression, antiviral immune response, and aging. DHX9 has become a relevant therapeutic target for diseases such as MM (multiple myeloma), showing promising results.
Contraindications: / The final concentration of DMSO in the reaction should not exceed 1%./ Some compounds may interfere with the results, depending on their color, spectral excitation and emission properties./ It is recommended that the compound alone is tested to determine any potential interference of the compound with the assay results.
Description: The DHX9 ATPase Activity Assay Kit is designed for the screening and profiling of DHX9 (DExH-box helicase 9) antagonists/inhibitors by monitoring their effect on the conversion of ATP to ADP using ADP-Glo™ as a detection reagent. ATP to ADP conversion occurs during DNA or RNA unwinding by the ATP dependent helicase domain of DHX9. DHX9 ATPase Activity Assay Kit comes in a convenient 96-well format, with contains enough purified recombinant DHX9 (amino acids 150-1150), ATP, DHX9 substrate, assay buffer and additives for 100 reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Target: DHX9
Uniprot: Q08211
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Castro J., et al., 2025 Cancer Res 85(4):758-776.
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