Target: Dityrosine
Conjugate: HRP
Product Type: Monoclonal
Clone Number: 10A6
Immunogen: Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
Purification: Protein G Purified
Storage Buffer: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
Concentration: 1 mg/ml
Specificity: Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
Scientific Background: ROS such as hydrogen peroxide (H2O2), superoxide, and hydroxyl radicals can react with both the backbone and the side chains of proteins, leading to backbone cleavage and side-chain modifications, respectively. Peroxidases, UV radiation, and hydroxyl radicals catalyze the formation of tyrosyl radicals which then react to form cross-links between proteins (1). This produces dityrosine, a metabolically stable biomarker of protein oxidation (2).
References: 1. Malencik, D. A., Anderson, S. R. (2003) Amino Acids. 25(3-4):233-247.2. Giulivi, C., Davies, K. J. A. (2001) J Biol Chem. 276, 24129-24136.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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