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Encompassing Amino Acids: 2-372
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performed in 50 mM Tris pH 7.4, 5 mM MgCl2, 1 mM DTT, 20 µM ATP, 0.2 mg/ml MRCL3 Peptide. Reaction was initiated with the addition of Mouse DRAK2 and was incubated at 30°C for 45 min. The luminescence was read with the Kinase Glo Max reagent (Promega).
Description: Mouse DRAK2, also known as DAP kinase-related apoptosis-inducing protein kinase 2, and death-associated protein kinase-related 2, GenBank Accession No. NM_133810, a.a. 2-372 (end), expressed in a baculovirus-infected Sf9 cell expression system, MW = 68 kDa.
Format: Aqueous buffer solution
Formulation: 50 mM HEPES, pH 7.5, 0.001% Brij-35, 1 mM DTT, 10 mM MgCl2, 150 mM KCl, 5% Glycerol, 0.1 mM EDTA, and 1mM Glutathione.
Genbank: NM_133810
Storage Stability: Aqueous buffer solution.
Tags: N-terminal GST-tag
Uniprot: Q8BG48
Biosafety Level: Not applicable (BSL-1)
References: 1. McGargill, M. et al., Immunity 21 (6): 781-791 (2004).
2. Mao, J. et al., J. Biol. Chem. 281: 12587-12595 (2006).
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