Description: This antibody reacts with the extracellular domain of EGFR and blocks the EGF/TGF induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, βcellulin and epiregulin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: B1D8
Immunogen: Microsomes from A431 cells.
Antibody Reactivity: EGFR
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Biol Chem (1984) 259(19): 11895-11900. (IP, functional studies)
- J Immunol Meth (2004) 287: 147-158. (Flow; epitope mapping)
Entrez Gene ID: 1956
Expected AB Applications: Flow, surface (published for clone)/Functional studies (published for clone)/IP (published for clone)
Z-Antibody Applications: Flow, surface (published)/Functional studies (published)/IP (published)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Does not react with mouse or rat, others not known/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Affinity Purification, for coupling, order Ab without BSA/Immunoprecipitation 1-2 ug/500 ug protein lysate/Optimal dilution for a specific application should be determined by user
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