Ep-CAM / CD326 (Epithelial Marker) (rVU-1D9), CF740 conjugate, 0.1mg/mL

Ep-CAM / CD326 (Epithelial Marker) (rVU-1D9), CF740 conjugate, 0.1mg/mL
SKU
BTMBNC741820-500
Packaging Unit
500 uL
Manufacturer
Biotium

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Description: This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM. It is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: rVU-1D9

Immunogen: Small cell lung carcinoma cells

Antibody Reactivity: CD326/Ep-CAM

References: Note: References for this clone sold by other suppliers may be listed for expected applications.

BMC Cancer (2017) 17: 811 (western)



Entrez Gene ID: 4072

Expected AB Applications: Exosome staining (verified for non-recombinant clone)/WB (published for clone)

Z-Antibody Applications: Exosome staining (expected)/Flow, surface (verified)/IF (verified)/IHC, FFPE (verified)/WB (published)

Verified AB Applications: Flow (surface) (verified)/IF (verified)/IHC (FFPE) (verified)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Immunohistology (formalin): 0.5-1 ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Western blotting 0.5-1 ug/mL/Optimal dilution for a specific application should be determined by user
More Information
SKU BTMBNC741820-500
Manufacturer Biotium
Manufacturer SKU BNC741820-500
Package Unit 500 uL
Quantity Unit STK
Reactivity Human
Clonality Recombinant
Application Immunofluorescence, Western Blotting, Flow Cytometry, Immunohistochemistry, Exosome Staining
Isotype IgG1 kappa
Host Mouse
Conjugate Conjugated, CF740
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