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Application: / Use as target cells in CAR-T or NK co-culture killing assays./ In vitro and in vivo bioluminescence imaging.
Background: B-lymphocyte antigen CD19 (Cluster of Differentiation 19), also known as B-Lymphocyte Surface Antigen B4 and CVID3, is a transmembrane protein expressed in follicular dendritic cells and all B lineage cells except plasma cells. CD19 plays two major roles in human B cells. It acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane and it works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. Due to its presence on all B cells, it is a biomarker for B lymphocyte development and lymphoma diagnosis and can be used as a target for leukemia immunotherapies. CD19-targeted therapies based on T cells that express CD19-specific chimeric antigen receptors (CARs) have been utilized for their antitumor abilities in patients with CD19+ lymphoma and leukemia, such as Non-Hodgkins Lymphoma (NHL), CLL (chronic lymphocytic leukemia) and ALL (acute lymphoblastic leukemia).
Description: Firefly Luciferase CD19 K562 Cell Line is a K562 cell line constitutively expressing full length human CD19 (cluster of differentiation 19) (also known as B4 or CVID3, Genbank #NM_001770) and firefly luciferase. This cell line has been validated by flow cytometry and luciferase activity.
Genbank: NM_001770
Host Cell Line: K562, human lymphoblast cell line, suspension.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Target: CD19
Uniprot: P15391
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
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