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Application: Useful for studying the expression of human GUCY2C on the cell surface by flow cytometry.
Assay Conditions: One million GUCY2C HEK293 cells (BPS Bioscience #83016) were blocked and stained with 1 µg of Anti-GUCY2C Antibody, PE-Labeled (BPS Bioscience #102744) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. GUCY2C HEK293 cells (green) were compared to parental HEK293 cells (blue). Y-axis is the cell count. X-axis is PE intensity.
Background: Human GUCY2C (guanylate cyclase 2c) is a membrane-bound receptor primarily expressed in intestinal epithelial cells and, to a lesser extent, in other tissues such as the liver and lung. GUCY2C plays a key role in regulating intestinal fluid and electrolyte homeostasis by catalyzing the conversion of GTP to cyclic GMP (cGMP) upon activation by endogenous ligands such as guanylin and uroguanylin, or by bacterial heat-stable enterotoxins. It is a crucial component of intestinal barrier function and fluid secretion. Dysregulation of GUCY2C signaling has been implicated in gastrointestinal disorders and colorectal cancer. Due to its restricted normal expression and overexpression in certain malignancies, GUCY2C has also merged as a promising biomarker and therapeutic target for colorectal cancer and other gastrointestinal tumors.
Clone Number: Monoclonal
Cross Reactivity: This antibody recognizes human GUCY2C. It has not been tested in other species.
Description: This anti-GUCY2C (guanylate cyclase 2c) antibody is a purified monospecific recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested by flow cytometry for binding to human GUCY2C on the surface of GUCY2CY HEK293 Cell Line (BPS Bioscience #83016). FluoSite™ is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.
Format: Aqueous buffer solution.
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.
Purification: Protein A affinity chromatography from HEK293 supernatants.
Purity: ≥85%
Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE.The antibody solution should be stored undiluted between 2°C and 8°C.
Target: GUCY2C
Uniprot: P25092
Warnings: Do not freeze. Protect from light.
Biosafety Level: Not applicable (BSL-1)
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