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Application: Useful for studying the expression of human PTK7 on the cell surface by flow cytometry.
Assay Conditions: One million PTK7 CHO cells (#82627) were blocked and stained with 1 µg of Anti-PTK7 Antibody, FITC-Labeled (#102766) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. PTK7 CHO cells (green) were compared to parental CHO-K1 cells (blue). Y-axis is the cell count. X-axis is FITC intensity.
Background: PTK7 (Protein Tyrosine Kinase 7) is a pseudokinase of the receptor tyrosine kinase (RTK) family of Wnt receptors and shares a common ligand (Wnt5a) with fellow pseudokinases ROR1 (receptor tyrosine kinase-like orphan receptor 1) and ROR2. Despite the lack of a functional kinase domain PTK7 still contributes to downstream signaling of Wnt5a through its interaction with LRP6 (low-density lipoprotein receptor-related protein 6) and FZD (frizzled) receptors. PTK7 expression contributes to oncogenic potential in a variety of solid tumors including ovarian, colorectal and breast cancers and neuroblastoma. The cell surface expression and oncogenic function of PTK7 in a variety of tumor types makes it a compelling target for both CAR (chimeric antigen receptor)-T cell and antibody based therapeutic development.
Cross Reactivity: This antibody recognizes human PTK7. It has not been tested in other species.
Description: This anti-PTK7 (Protein Tyrosine Kinase 7) antibody is a purified monospecific recombinant antibody, which is labeled with Fluorescein isothiocyanate (FITC). This antibody has been tested by flow cytometry for binding to human PTK7 on the surface of PTK7 CHO Cell Line (BPS Bioscience #82627). FluoSite™ is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.
Format: Aqueous buffer solution
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.
Host Cell Line: HEK293
Purification: Protein A affinity chromatography from HEK293 supernatants.
Purity: ≥85%
Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.
Target: PTK7
Uniprot: Q13308
Unit Definition: Antibody
Warnings: Do not freeze. Protect from light.
Biosafety Level: Not applicable (BSL-1)
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