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Application: Useful for studying the expression of human PTK7 on the cell surface by flow cytometry.
Assay Conditions: One million PTK7 CHO cells (#82627) were blocked and stained with 1 µg of Anti-PTK7 Antibody, PE-Labeled (#102763) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. PTK7 CHO cells (green) were compared to parental CHO-K1 cells (blue). Y-axis is the cell count. X-axis is PE intensity.
Background: PTK7 (Protein Tyrosine Kinase 7) is a pseudokinase of the receptor tyrosine kinase (RTK) family of Wnt receptors and shares a common ligand (Wnt5a) with fellow pseudokinases ROR1 (receptor tyrosine kinase-like orphan receptor 1) and ROR2. Despite the lack of a functional kinase domain PTK7 still contributes to downstream signaling of Wnt5a through its interaction with LRP6 (low-density lipoprotein receptor-related protein 6) and FZD (frizzled) receptors. PTK7 expression contributes to oncogenic potential in a variety of solid tumors including ovarian, colorectal and breast cancers and neuroblastoma. The cell surface expression and oncogenic function of PTK7 in a variety of tumor types makes it a compelling target for both CAR (chimeric antigen receptor)-T cell and antibody based therapeutic development.
Cross Reactivity: This antibody recognizes human PTK7. It has not been tested in other species.
Description: This anti-PTK7 (Protein Tyrosine Kinase 7) antibody is a purified monospecific recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested by flow cytometry for binding to human PTK7 on the surface of PTK7 CHO Cell Line (BPS Bioscience #82627). FluoSite™ is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.
Format: Aqueous buffer solution
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.
Host Cell Line: HEK293
Purity: ≥85%
Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.
Target: PTK7
Uniprot: Q13308
Unit Definition: Antibody
Warnings: Do not freeze. Protect from light.
Biosafety Level: Not applicable (BSL-1)
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