Products from BPS Bioscience require a minimum order value above 400€
Application: Useful for studying the expression of human TROP2 on the cell surface by flow cytometry.
Assay Conditions: One million TROP2-CHO-K1 cells (#78099) were blocked and stained with 1 µg of Anti-TROP2 Antibody, PE-Labeled (#102773) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. TROP2-CHO-K1 cells (green) were compared to parental CHO-K1 cells (blue). Y-axis is the cell count. X-axis is PE intensity.
Background: Trophoblast cell-surface antigen 2 (TROP-2), also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein that is highly expressed in a variety of solid cancers yet has low expression in normal cells. Through a variety of signaling pathways, TROP2 regulates cancer growth and metastasis and is a favorable target for antibody drug conjugates (ADC) and Adoptive Cell Therapy.
Cross Reactivity: This antibody recognizes human TROP2. It has not been tested in other species.
Description: This anti-TROP2 (Trophoblast cell-surface antigen 2) antibody is a purified monospecific recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested by flow cytometry for binding to human TROP2 on the surface of TROP2-CHO-K1 Cell Line (BPS Bioscience #78099). FluoSite™ is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.
Format: Aqueous buffer solution
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.
Host Cell Line: HEK293
Purification: Protein A affinity chromatography from HEK293 supernatants.
Purity: ≥85%
Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.
Tags: PE is an oligomeric protein complex (270 kDa) from red algae that exhibits intensely bright red-orange fluorescence with excitation peak at 566 nm and an emission peak at 574 nm. The complex consists of six heterodimers, α subunit (18 kDa) and β-subunit (20 kDa), and an additional γ-subunit (34 kDa).
Target: TROP2
Uniprot: P09758
Warnings: Do not freeze. Protect from light
Biosafety Level: Not applicable (BSL-1)
English
Deutsch
