Clone: L50-823.
Background: GATA3 (GATA binding protein 3 to DNA sequence [A/T]GATA[A/G]) is a zink finger transkription faktor and plays an important role in cell proliferation, development and differentiation in many tissues and cell types. Among other roles, GATA3 is involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the proliferation of breast cancer. GATA3 is primarily over-expressed in breast and urothelial carcinomas and only rarely found in tumors of other organs. Therefore anti-GATA3 has been used for diagnosis of unknown primary carcinoma, when carcinomas of the breast or bladder are a possibility. In a clinical study it was demonstated that GATA-3 stained 84% of urothelial carcinoma and 94% Mammacarcinoma (2). However, another study demonstates positivity for GATA3 in a number of other tumours (3), so that it is indicated to use more specific markers for differential diagnosis. For mamma carcinoma Mammaglobin and GCDFP should be used in addition to GATA3. GATA3; Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3.
Positive Control: Breast carcinoma, Urothelial carcinoma (nuclear staining).
Immunogen: Conserved peptide between the GATA trans-activation and DNA-binding domain.
Purification Method: Purified antibody in PBS with 0.2% BSA and 15 mM sodium azide.
References: 1. Higgins JP et al. Placental S100 (S100P) and GATA3: markers for transitional epithelium and urothelial carcinoma discovered by complementary DNA mircoarray. Am J Surg Pathol. 2007; 31:673-680. 2. Liu H et al. Immunohistochemical evaluation of GATA3 expression in tumors and normal tissues. Am J Clin Pathol. 2012; 138:57-64. 3. Miettinen M et al. GATA 3 – A multispecific but potentially useful marker in surgical pathology – a systematic analysis of 2500 epithelial and non-epithelial tumors. Am J Surg Pathol. 2014; 38:13-22.
UniProt: P23771 (GATA3_HUMAN).
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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