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Application: Study and screen compounds that inhibit the binding of GDF15 to GFRAL for drug discovery in high throughput screening (HTS) applications.
Background: Growth and differentiation factor 15 (GDF15) is a cytokine, member of the TGF-β superfamily, and binds to the receptor Glial-derived neurotrophic factor-family receptor α-like (GFRAL). GDF15 is not normally expressed and is thought to be induced at times of stress. GDF15 is considered a biomarker for inflammation and adverse cardiovascular events. Elevation in GDF15 expression levels reduces food intake and body mass through binding to GFRAL and recruitment of the tyrosine kinase RET in the hindbrain. Due to the role of GDF15 in suppressing inflammation and appetite, it is a promising target to treat metabolic diseases including obesity, type 2 diabetes, non-alcoholic fatty liver disease, cardiovascular disease, and cancer cachexia.
Contraindications: This kit is compatible with up to 1% final DMSO concentration.
Description: The GDF15: GFRAL [Biotinylated] Inhibitor Screening Chemiluminescent Assay Kit is an ELISA designed to measure the binding between GDF15 (Growth and differentiation factor 15) and GFRAL (Glial-derived neurotrophic factor-family receptor α-like) proteins for screening and profiling applications. This kit comes with enough purified GDF15 (amino acids 197-308) and biotinylated GFRAL (amino acids 19-351(end)), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.A 96-well plate is coated with GDF15 protein. After blocking, biotinylated GFRAL is added in an optimized assay buffer. Next, unbound biotinylated GFRAL is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of GFRAL binding to GDF15.Need us to run inhibitor screens or profile your compounds? Check out our Immunotherapy Biochemical Screening Services.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Target: GDF15, GFRAL
Uniprot: Q99988, Q6UXV0
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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