Background: The antibody is well suited for the detection of glucagon in tissue sections of brain, pancreas and stomach. Glucagon is a polypeptide which is produced by s.c. A-cells of the pancreatic islets. It is the counterpart of insulin in the regulation of blood sugar levels. Except of the pancreas, glucagon exists also in endocrine cells of the stomach and in neurons of the brain. Frequently it has been found in pancreatic tumours and in rectal carcinoids, which can be responsible for glucagonoma syndromes. Glucagon.
Positive Control: Pancreas.
Immunogen: Porcine pancreatic Glucagon-BSA conjugate.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
References: 1. Böttcher G, Ahrén B, Lundquist I, Sundler F. (1989) Peptide YY: intrapancreatic localization and effects on insulin and glucagon secretion in the mouse. Pancreas. 4(3):282-8. 2. Riedel MJ, Asadi A, Wang R, Ao Z, Warnock GL, Kieffer TJ. (2012) Immunohistochemical characterisation of cells co-producing insulin and glucagon in the developing human pancreas. Diabetologia. 55(2):372-81. 3. Sebastian Barg, Juris Galvanovskis, Sven O. Göpel, Patrik Rorsman, and Lena Eliasson (2000) Tight Coupling Between Electrical Activity and Exocytosis in Mouse Glucagon-Secreting ?-Cells DIABETES, VOL. 49;1500-1510 4. Anna Wendt, Bryndis Birnir, Karsten Buschard, et al. (2004) Glucose Inhibition of Glucagon Secretion From Rat ?-Cells Is Mediated by GABA Released From Neighboring ß-Cells. Diabetes Vol. 53; 1038-1045.
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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