Shelf life (days): 365.0
Formulation: 100 µg of protein G-purified IgG
Immunogen: Produced as a recombinant protein expressed in HEK293-derived cells using an engineered construct developed based on the Histone H3 (Citrullinated R2 + R8 + R17) Monoclonal Antibody (Clone 11D3)
Clone Designation: 11D3
Target specificity: Human
Cross Reactivity: + Citrullinated histone H3; - Histone H3; - Other citrullinated proteins
Uniprot ID: P68431
Formula Weight: 0.0
Notes: Histone H3 is a nuclear protein and a component of the nucleosome core that is essential for organizing genomic DNA in eukaryotic nuclei.{56209} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications, including citrullination. Histone H3 is subject to citrullination of arginine residues by peptidyl arginine deiminase 4 (PAD4) at positions 2, 8, 17, and 26, and this citrullination blocks methylation of these residues by protein-arginine methyltransferase 4 (PRMT4) and induces transcriptional repression of target genes. Histone H3 is subject to citrullination by protein-arginine deiminase 4 (PAD4) at arginine 2 (R2), R8, and R17 during neutrophil extracellular trap formation (NETosis).{18136} Citrullination of histone H3 at these residues reduces the positive charge of histone H3 and leads to decondensed chromatin, which is expelled into the extracellular space when the neutrophil membrane ruptures.{65427,18136} Citrullinated histone H3 is a component of neutrophil extracellular traps (NETs) that acts as an autoantigen to induce production of anti-citrullinated protein antibodies associated with various diseases such as sepsis, multiple sclerosis, rheumatoid arthritis, and multiple myeloma.{56209,30129,35770,46253} Increased serum levels of histone H3 citrullinated at R2, R8, and R17 have been found in patients with asthma and in septic shock patients where they are positively associated with a sequential organ failure assessment (SOFA) score and poor survival.{65428,65429} Sputum levels of histone H3 citrullinated at R2, R8, and R17 are increased in patients with rheumatoid arthritis, as well as in individuals identified as at risk for developing rheumatoid arthritis based on their seropositivity for IgA anti-citrullinated protein antibodies (ACPAs).{65430} Cayman’s Citrullinated Histone H3 (R2 + R8 + R17) Recombinant Monoclonal Antibody, which contains a mouse IgG isoform, was produced as a recombinant protein expressed in HEK293-derived cells using an engineered construct developed based on the Histone H3 (Citrullinated R2 + R8 + R17) Monoclonal Antibody (Clone 11D3). This antibody can be used for ELISA and Western blot (WB) applications.
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