Clone: HP-6054.
Background: The anti-lambda antibody may be used for the detection of abnormal immunoglobulin production, e.g.. monoclonal gammopathy with increased production of both free and bound lambda chains. In this context the differentiation from kappa light chains or heavy chains is of importance. The antibody stains B-cells in lymphoidal tissue and peripheral blood as well as plasma cells expriming lambda chains (especially useful in formaldehyde-fixed paraffin sections). Reed-Sternberg-Cells may be stained if exogenous Ig has been absorbed. The antibody may be helpful for the detection of the monoclonal nature of lymphoidal neoplasms. Immunoglobulins are composed of heavy and light chains and the light chains may be of the kappa or lambda type. In humans app. 35% of light chains are lambda chains. In gammopathy these light chains can occur in high concentration as free lambda chains. Human lambda light chain (free and bound), no cross-reactivity with kappa chains.
Positive Control: Tonsil.
Immunogen: Purified human myeloma-IgG polyaminostyrene conjugate.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
References: 1. N. Leung, S. V. Rajkumar (2007) Renal Manifestations of Plasma Cell Disorders. American Journal of Kidney Diseases 50; 155-165 2. Markowitz GS (2004) Dysproteinemia and the kidney. Adv Anat Pathol 11:49-63.
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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