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Application: / Screen IL-17A or IL-17RA antibodies./ Monitor IL-17A signaling pathway activity.
Background: IL-17A, originally known as CTLA-8 (cytotoxic T lymphocyte-associated antigen 8), is a cytokine crucial in infection and inflammatory processes. It is the most studied of the IL-17 family, which consists of six structurally related proteins: IL-17A to IL-17F. It is produced in macrophages, dendritic cells, and γδ-T cells. It signals via the IL-17R (IL-17 receptor) family of proteins, which includes the subunits IL-17RA-E, and are single-pass transmembrane receptors. Signaling occurs via a heterodimeric receptor typically composed of IL-17RA and a second chain. Upon binding of IL-17 to the receptor, Act1 (Act 1 adaptor protein) is activated and TRAF6 (TNF receptor associated factor 6) is recruited. Ubiquitinated TRAF6 leads to the expression of inflammatory related genes, via the NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) pathway. IL-17 dysregulation can result in psoriasis and other auto-immune disorders. The use of monoclonal antibodies able to block IL-17R have proved beneficial in the treatment of plaque psoriasis and rheumatic disorders, and bimekizumab, brodalumab, secukinumab and ixekizumab are already approved by the FDA.
Description: IL-17A Responsive Luciferase Reporter HEK293 Cell Line is a HEK293 cell designed to monitor IL-17A (interleukin-17 A) activity through the endogenous IL-17 receptor complex. It contains a firefly luciferase reporter driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by IL-17A, NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter. The cell line has been functionally validated in response to human IL-17A as well as by inhibition by an anti-IL-17 receptor and anti-IL-17 antibodies.
Host Cell Line: HEK293, epithelial-like cells, adherent.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Target: IL-17A, IL-17RA
Uniprot: Q16552
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: Pessara U. and Koch N., 1990 Mol Cell Biol. 10(8): 4146-4154.Baeuerle P.A., 1998 Curr Biol. 8(1): R19-R22.Takada Y., et al., 2005 J Biol Chem. 280(17): 17203-17212.
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