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Application: Study and screen compounds that inhibit the binding of IL-31 to IL-31RA for drug discovery in high throughput screening (HTS) applications.
Background: IL-31 (interleukin 31) is a four-helix bundle proinflammatory cytokine preferentially produced by T helper type 2 (TH2) cells. IL-31 regulates cell differentiation, cell proliferation, and immune responses, serving as a neuroimmune link between TH2 cells and sensory neurons in generating a T cell-mediated inflammatory itch. The IL-31 receptor complex, composed of IL-31RA and OSMR (human oncostatin M receptor beta), is expressed by various epithelial and immune cells as well as dorsal root ganglia sensory neurons. IL-31 interacts with the IL-31 receptor complex, activating Janus kinases JAK1 and JAK2. This activation leads to the phosphorylation of signal transducer and activator of transcription (STAT) molecules, mainly STAT3, which upregulate the genes responsible for the IL-31 induced itch sensation. Often referred to as the itchy cytokine, increased expression of IL-31 or its receptor IL-31RA is correlated with alopecia, skin lesions, airway hypersensitivity, and particularly pruritic disorders such as atopic dermatitis. IL-31 and other TH2 related cytokines (including IL-4, IL-13 and TSLP (thymic stromal lymphopoietin) play an important role in the pathogenesis of a variety of inflammatory and allergic diseases and have become popular for therapeutic development. The IL-31RA targeting antibody Nemolizumab has been FDA-approved for the treatment of atopic dermatitis.
Description: The IL-31: IL-31RA [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between IL-31 and IL-31RA protein for screening and profiling applications. This kit comes with enough purified IL-31 (amino acids 24-164(end)) and biotinylated IL-31RA (amino acids 20-519), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. IL-31: IL-31RA [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit schematic. A 96-well plate is coated with IL-31 protein. After coating and blocking, biotinylated IL-31RA is added in an optimized assay buffer. Next, unbound biotinylated IL-31RA is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of IL-31RA binding to IL-31.Need us to run inhibitor screens or profile your compounds that block the binding of IL31to IL31RA? Check out our Immunotherapy Biochemical Screening Services.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: This kit comes with enough purified IL-31 (amino acids 24-164(end)) and biotinylated IL-31RA (amino acids 20-519), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.
Target: IL-31, IL-31Ra
Uniprot: Q8NI17
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Nemmer J., et al., 2021 Front. Med. 8: 639097.Takahashi S., et al., 2023 Cell Rep. 42(12): 113433
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