Description: IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T-cells, B-cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Rattus norvegicus (laboratory rat), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: MP5-20F3
Immunogen: Recombinant mouse IL-6 protein
Antibody Reactivity: IL-6
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Immunol (1990) 145(12): 4185-4191. (functional studies)
- J Immunol (2013) 190:703-711. (functional studies; Flow)
Entrez Gene ID: 16193 (Mouse)
Expected AB Applications: Flow (intracellular) (published for clone)/Functional studies (published for clone)
Z-Antibody Applications: Flow, intracellular (published)/Functional studies (published)
Antibody Application Notes: Flow cytometry: 0.5-1 ug/million cells in 0.1 mL/Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Does not react with human or rat; others not known/Neutralization Studies order Ab without azide/Optimal dilution for a specific application should be determined by user
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