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Application: Study and screen compounds that inhibit the binding of IL1RL1 to IL33 for drug discovery in high throughput screening (HTS) applications.
Background: IL33 is a cytokine of the IL1 family, that is released in response to external triggers, such as trauma, allergen exposure and infections. IL33 can be either on its reduced or oxidized state, which use different signaling pathways. When in its reduced state (IL33red) it binds to IL1RL1 ((interleukin 1 receptor like 1), also named IL33R or ST2 (receptor serum-stimulated 2). IL1RL1 exists as both a membrane bound protein or a truncated soluble form. Binding of IL33 to IL1RL1 initiated a signaling pathway that involves NF-κF (nuclear factor kappa-light-chain-enhancer of activated B cells) and MAPK (mitogen-activated protein kinase), and results in release of pro-inflammatory cytokine and chemokines. The soluble form of IL1RL1 can act as a decoy receptor for IL33. Dysregulation in the levels of IL33 is implicated in the pathology of inflammatory and infectious diseases, including COVID-19, COPD (chronic obstructive pulmonary disease) and asthma. The involvement of IL33 in these diseases has made it a high value therapeutic target, with strategies such as antibodies being developed to inhibit the binding of IL33 to its receptor. One example is tozorakimab (MEDI3506), from AstraZeneca, currently being evaluated as treatment for COPD. Further studies on IL33 and its receptor will likely result in new therapies for IL33 related disorders.
Contraindications: This assay kit is compatible with up to 1% final DMSO concentration.
Description: The IL1RL1: IL33 [Biotinylated] Inhibitor Screening Chemiluminescent Assay Kit is an ELISA-based assay designed to measure the binding between IL-1RL1 (interleukin 1 receptor like 1 or IL33R) and IL-33 protein for screening and profiling applications. This kit comes with enough purified IL1RL1 (amino acids 19-328) and biotinylated IL33 (amino acids 109-270), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. IL1RL1: IL33 [Biotinylated] Inhibitor Screening Chemiluminescent Assay Kit schematic. A 96-well plate is coated with IL1RL1 protein. After coating and blocking, biotinylated IL33 is added in an optimized assay buffer. Next, unbound biotinylated IL33 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of IL33 binding to IL1RL1.Need us to run inhibitor screens or profile your compounds that block the binding og IL1RL1 to IL33? Check out our Immunotherapy Screening Services.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Target: IL-1a, IL-1b, IL-33
Uniprot: IL1RL1:Q01638; IL33: O95760
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: England E., et al., 2023 Scientific Reports 13:9825.
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